The Role of In Vivo and Ex Vivo Diagnostic Tools in Severe Delayed Immune-Mediated Adverse Antibiotic Drug Reactions

A Copaescu; E Mouhtouris; S Vogrin; F James; KYL Chua; NE Holmes; A Douglas; MA Slavin; H Cleland; C Zubrinich; AK Aung; MSY Goh; EJ Phillips; JA Trubiano

Journal article

The Role of In Vivo and Ex Vivo Diagnostic Tools in Severe Delayed Immune-Mediated Adverse Antibiotic Drug Reactions

A Copaescu, E Mouhtouris, S Vogrin, F James, KYL Chua, NE Holmes, A Douglas, MA Slavin, H Cleland, C Zubrinich, AK Aung, MSY Goh, EJ Phillips, JA Trubiano

Journal of Allergy and Clinical Immunology in Practice | ELSEVIER | Published : 2021

DOI: 10.1016/j.jaip.2020.12.052

Abstract

Background: The use of in vivo and ex vivo diagnostic tools for delayed immune-mediated adverse drug reactions is currently ill defined. Objective: To determine whether the combination of skin testing and/or IFN-γ enzyme-linked immunoSpot assay (ELISpot) can aid diagnosis of these allergy phenotypes. Methods: Patients with antibiotic-associated severe delayed immune-mediated adverse drug reaction hypersensitivity, including Stevens-Johnson syndrome and toxic epidermal necrolysis, drug reaction with eosinophilia and systemic symptoms (DRESS), acute generalized exanthematous pustulosis, generalized bullous fixed drug eruption, and severe maculopapular exanthema, were prospectively recruited. I..

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University of Melbourne Researchers

Sara Vogrin Author

Kyra Chua Author

Natasha Holmes Author

Monica Slavin Author

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Grants

Awarded by National Institutes of Health

Funding Acknowledgements

E.J.P. receives support National Institutes of Health, United States from grant numbers 1P50GM115305, R21AI139021, 1 R01 HG010863, and 1R01AI152183. J.A.T. was supported by the Austin Medical Research Foundation, Australia and by a National Health and Medical Research Council postgraduate scholarship (grant no. GNT 1139902) and Royal Australian College of Physicians Research Establishment Fellowship.